Signalling

Part:BBa_I756035:Design

Designed by: Jennifer Lei   Group: iGEM07_Bay_Area_RSI   (2007-10-25)


CD18-Cub-mlexA-linker-VP16


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 3118
    Illegal XbaI site found at 3379
    Illegal PstI site found at 1167
    Illegal PstI site found at 1419
    Illegal PstI site found at 1938
    Illegal PstI site found at 1960
    Illegal PstI site found at 3162
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 3118
    Illegal PstI site found at 1167
    Illegal PstI site found at 1419
    Illegal PstI site found at 1938
    Illegal PstI site found at 1960
    Illegal PstI site found at 3162
    Illegal NotI site found at 2504
    Illegal NotI site found at 3144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 3118
    Illegal BglII site found at 3048
    Illegal BamHI site found at 177
    Illegal BamHI site found at 3128
    Illegal XhoI site found at 3151
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 3118
    Illegal XbaI site found at 3379
    Illegal PstI site found at 1167
    Illegal PstI site found at 1419
    Illegal PstI site found at 1938
    Illegal PstI site found at 1960
    Illegal PstI site found at 3162
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 3118
    Illegal XbaI site found at 3379
    Illegal PstI site found at 1167
    Illegal PstI site found at 1419
    Illegal PstI site found at 1938
    Illegal PstI site found at 1960
    Illegal PstI site found at 3162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1373
    Illegal SapI site found at 2321


Design Notes

none


Source

kpn1 (1-6bp)

kozak (7-12bp)

CD18 from Integrin beata2-mYFP, Kim, M et al. Science 2003, 5640:1720-5 (13-2319bp)

BspQ1 linker (2320-2361bp)

Cub seembled from oligos (2362-2487bp)

linker (2488-2511bp)

mLexA from PEG202 (2512-3201bp)

linker (3202-3234bp)

VP16 from HSV1 helper virus (Salposky lab, Stanford) (3235-3372bp)

Stop Stop (3373-3378bp)

Xba1 (3379-3384bp)

References